![]() To date, the only procedure for the rapid intraoperative assessment of BC-SLN metastasis is frozen-section (FS) analysis, in which 4 μm-thick FS sections of the intraoperatively dissected SLN with hematoxylin–eosin (H&E) staining are sent for immediate histopathological analysis to check for the presence of metastatic cancer cells. Thus, OCMIS is a useful noninvasive image-assisted tool for breast cancer metastasis assessment based on SLN images. Compared with gold-standard paraffin-sectioned H&E images, the specificity, sensitivity, and accuracy obtained with the frozen-sectioned H&E images ( n = 85) of the specimens were the same as those obtained with the p-H&E images ( n = 95). Specimens from 29 patients at Wanfang Hospital were collected after excision and were analyzed at the pathology laboratory, and a fluorescence-in-built optical coherence microscopic imaging system (OCMIS) was then used to visualize the pseudo-H&E (p-H&E) images of the SLNs for intraoperative breast cancer metastasis assessment, and the specificity, sensitivity, and accuracy were 100%, 88.9%, and 98.8% ( n = 83), respectively. Optical-sectioned H&E images provide a nondestructive method for obtaining the insight en face image near the attached surface of the dissected specimen, preventing the freezing problem of fatty tissue. After frozen sectioning, the sliced fatty region of the frozen-sectioned specimen is easily dropped because of different freezing points for fatty tissues and other tissues. ![]() Frozen-sectioned hematoxylin–eosin (H&E) image evaluation is the current method for intraoperative breast cancer metastasis assessment through ex vivo sentinel lymph nodes (SLNs).
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |